BIX 02565 is primarily utilized in research settings as a potent and selective inhibitor of Rho-associated protein kinase (ROCK). It is commonly employed in studies focused on understanding the role of ROCK signaling pathways in various cellular processes, including cytoskeletal organization, cell migration, and apoptosis. Researchers use it to investigate its effects on conditions such as cardiovascular diseases, neurological disorders, and cancer, where ROCK activity plays a critical role. Additionally, BIX 02565 is valuable in exploring potential therapeutic strategies targeting ROCK-mediated mechanisms in these diseases. Its application extends to in vitro and in vivo models, aiding in the development of novel pharmacological interventions.