ELR510444 is a potent and selective small-molecule inhibitor of TBK1 (TANK-binding kinase 1) and IKKε (IκB kinase epsilon), key regulators in innate immune signaling and NF-κB pathways. It is primarily utilized in biomedical research to study cellular signaling, particularly in contexts of inflammation, antiviral responses, and oncogenesis. As a selective inhibitor, it enables precise investigation of kinase-dependent molecular interactions and pathway dysregulation. This compound is employed in experimental studies elucidating disease mechanisms involving abnormal cell proliferation or immune signaling errors, such as cancer and inflammatory disorders. In drug discovery, it validates therapeutic targets by blocking these pathways and assessing cellular responses. ELR510444 also supports preclinical efficacy and safety evaluations for novel treatments.