TRC051384 is primarily used in research settings for its potent activity as a selective inhibitor of sphingosine kinase 2 (SphK2), an enzyme involved in sphingolipid metabolism. By inhibiting SphK2, it modulates the balance of bioactive sphingolipids such as sphingosine-1-phosphate (S1P) and ceramide, which play critical roles in cell survival, proliferation, and apoptosis. This makes TRC051384 a valuable tool compound in studies related to cancer, inflammation, and neurodegenerative diseases. It has demonstrated anti-proliferative effects in various cancer cell lines, particularly in hematological malignancies, and is often used to probe the therapeutic potential of targeting the sphingolipid pathway. Additionally, it has been employed in preclinical models to investigate mechanisms of drug resistance and to evaluate combination therapies with other anticancer agents.