TH-257 is primarily used as a selective inhibitor in biochemical research, particularly in studies involving kinase pathways. It demonstrates potent activity against specific tyrosine kinases, making it a valuable tool for investigating signal transduction mechanisms in cellular proliferation and inflammation. Due to its selectivity, TH-257 is employed in early-stage drug discovery to validate therapeutic targets, especially in oncology and autoimmune disease research. It is also utilized in in vitro assays to modulate enzyme activity and assess downstream biological effects, supporting the development of targeted therapies. Its application extends to pharmacokinetic and pharmacodynamic studies where precise control over kinase inhibition is required.