Thiazovivin is primarily used in stem cell research to improve the efficiency of induced pluripotent stem cell (iPSC) generation. It acts as a potent small-molecule inhibitor of Rho-associated protein kinase (ROCK), helping to enhance cell survival, particularly during the critical stages of single-cell passaging and reprogramming. By reducing apoptosis and promoting cell attachment, Thiazovivin allows for higher yields of viable stem cells without the need for feeder layers or complex culture conditions. It is commonly added to culture media to maintain pluripotency and support the expansion of stem cell populations in regenerative medicine and disease modeling applications.