SL-327 is primarily used in scientific research as a selective inhibitor of the MEK/ERK signaling pathway. It is commonly employed to study cellular processes such as proliferation, differentiation, and apoptosis. Due to its ability to cross the blood-brain barrier, SL-327 is especially valuable in neuroscience research, where it helps investigate the role of ERK activation in learning, memory formation, and neurodegenerative conditions. It has been utilized in animal models to explore potential therapeutic strategies for diseases involving aberrant MAP kinase signaling, including certain cancers and neurological disorders. Its application remains restricted to preclinical studies, mainly in vitro and in vivo experimental models.