SNS-314 Mesylate is primarily used in research settings as a potent and selective Aurora B kinase inhibitor. It plays a key role in studies related to cell division and mitotic regulation, making it a valuable tool for investigating cancer cell proliferation. By inhibiting Aurora B, it induces polyploidy and apoptosis in tumor cells, which supports its use in oncology research, particularly in the development of targeted anti-cancer therapies. It has been utilized in preclinical studies to assess the effects of Aurora kinase inhibition on tumor growth and chemotherapy response. Due to its mechanism, it also serves as a reference compound in screening new kinase inhibitors and understanding mitotic checkpoint control.