SMI-16a is primarily used as a selective inhibitor in biochemical research, particularly targeting specific signaling pathways involved in cellular proliferation and inflammation. It has been applied in studies related to immune modulation, showing potential in suppressing overactive immune responses associated with autoimmune diseases. Researchers utilize SMI-16a to probe kinase-dependent pathways, helping to identify therapeutic targets in cancer and chronic inflammatory conditions. Its application in cell-based assays allows for the investigation of protein-protein interactions and signal transduction mechanisms, making it a valuable tool in drug discovery and molecular pharmacology.