Used as a potent and selective inhibitor in biochemical research, particularly targeting enzymes involved in inflammatory and oncogenic pathways. Its rigid, polycyclic structure enables high binding affinity to specific protein targets, making it valuable in the development of therapeutic agents for cancer and autoimmune diseases. Commonly employed in cell-based assays and in vivo studies to modulate signaling pathways such as NF-κB and MAPK. Also utilized as a molecular probe to study protein-protein interactions and enzyme kinetics due to its stability and specificity. Its quinoline and isoindole moieties contribute to favorable pharmacokinetic properties, supporting its use in preclinical drug discovery. Additionally, studies have evaluated its ability to cross the blood-brain barrier, suggesting potential applications in treating central nervous system tumors, such as brain cancer, due to its structural suitability for BBB penetration.