RO-7 is primarily used in research settings for its potent inhibitory effects on specific kinase enzymes involved in cellular signaling pathways. It has shown promise in preclinical studies as a candidate for targeting certain types of cancer, particularly those driven by dysregulated protein phosphorylation. Due to its ability to cross cell membranes efficiently, RO-7 is valued in cellular assays for modulating intracellular kinase activity with high specificity. It is also employed in neuroscience research to study synaptic plasticity and neuronal survival pathways. Its application remains largely confined to laboratory and experimental models, where it aids in validating drug targets and understanding disease mechanisms.