Ro 31-8220 is widely used in biomedical research as a potent and selective inhibitor of protein kinase C (PKC). It is commonly employed to study cellular signaling pathways involving PKC, helping researchers understand the role of this enzyme in processes such as cell proliferation, differentiation, and apoptosis. Due to its ability to cross cell membranes effectively, Ro 31-8220 is useful in cell-based assays to suppress PKC activity, allowing for the investigation of PKC-dependent mechanisms in cancer, neurodegenerative diseases, and cardiovascular conditions. It also serves as a tool compound in studies exploring the therapeutic potential of kinase inhibition, particularly in inflammation and immune responses. Its selectivity makes it valuable for distinguishing PKC-mediated effects from those of other kinases in complex biological systems.