Reversine is primarily used in stem cell research and regenerative medicine due to its ability to induce dedifferentiation of mature cells into a pluripotent state. It acts as a small molecule inhibitor targeting key kinases involved in cell cycle regulation and differentiation, such as Aurora kinase and JNK. This property makes it valuable for reprogramming differentiated cells, like fibroblasts, back into progenitor-like cells without the need for genetic manipulation. It is also employed in studies focused on tissue regeneration, particularly in cartilage and skeletal muscle repair, where it promotes the formation of multipotent progenitor cells capable of differentiating into various lineages. Additionally, reversine is used as a tool compound in cancer research to investigate cell cycle arrest and apoptosis in tumor cells, given its ability to interfere with mitotic progression. Its role in chemical-induced cell fate modulation continues to support drug discovery and development of novel therapeutic strategies.