Used as a selective inhibitor of Rho-associated protein kinase (ROCK) in research settings, particularly in cell biology and neuroscience. Helps in studying cellular processes such as cytoskeletal organization, cell migration, and neurite outgrowth. Commonly applied in experiments involving stem cell differentiation, where it enhances cell survival and cloning efficiency. Also utilized in studies related to smooth muscle contraction, vascular tone, and potential regenerative medicine applications due to its ability to modulate cellular morphology and adhesion.