NG25 is primarily used as a selective inhibitor of the transforming growth factor-beta (TGF-β) receptor kinase. It plays a significant role in research settings focused on cell signaling pathways, particularly in studies related to fibrosis, cancer progression, and epithelial-mesenchymal transition (EMT). By blocking TGF-β signaling, NG25 helps researchers investigate the functional impact of this pathway in various cellular models. It is also employed in developmental biology and regenerative medicine research to modulate differentiation and maintain cell pluripotency. Its application extends to inflammation studies, where TGF-β pathway regulation influences immune cell behavior. Due to its specificity and cell permeability, NG25 is valued in pharmacological experiments aiming to dissect kinase-dependent mechanisms without broad off-target effects.