Used in research to selectively activate engineered kinases in chemical genetic studies. Enables control of protein activity in cell signaling pathways with high temporal precision. Commonly applied in studies of cell cycle regulation, signal transduction, and kinase function in live cells. Also utilized in inducible gene expression systems and to study protein-protein interactions in mammalian and yeast models. Its inertness to wild-type proteins minimizes off-target effects, making it valuable for precise biological manipulation.