KN-93 is widely used in biomedical research to investigate the role of calcium/calmodulin-dependent protein kinase II (CaMKII) in cellular signaling pathways. It acts as a selective inhibitor of CaMKII, making it a valuable tool for studying processes such as neuronal plasticity, cardiac function, and immune responses where CaMKII activity is critical. Researchers apply KN-93 in models of heart disease to explore arrhythmias and hypertrophy, as CaMKII overactivation is linked to these conditions. In neuroscience, it helps examine synaptic transmission, learning, and memory by blocking CaMKII-mediated phosphorylation events. KN-93 is also used in studies of inflammation and apoptosis, where it can modulate cell survival pathways. Due to its cell-permeable nature, it is effective in both in vitro and in vivo experimental systems, allowing for broad application across physiological research areas.