Used in research as a selective inhibitor of IKKε (IκB kinase epsilon), IMD 0354 is widely applied in studies related to inflammation and immune response modulation. It effectively suppresses the activation of NF-κB, a key transcription factor involved in the expression of pro-inflammatory cytokines, making it a valuable tool in investigating inflammatory diseases such as arthritis, asthma, and inflammatory bowel disease. Additionally, IMD 0354 has been explored in cancer research due to its ability to induce apoptosis and inhibit proliferation in certain tumor cell lines by blocking NF-κB signaling pathways. Its application extends to virology, where it helps study viral replication mechanisms that depend on host inflammatory responses. Due to its cell permeability and relatively low toxicity in experimental models, IMD 0354 is commonly used in in vitro and in vivo studies to dissect signaling pathways and evaluate potential therapeutic targets.