Used as a key intermediate in the synthesis of various pharmaceuticals, including kinase inhibitors, anti-inflammatory agents, and antimicrobials targeting cancer and neurological disorders. It facilitates the construction of benzodiazine-1,4 (quinazoline) heterocyclic scaffolds due to its reactive anhydride group and hydroxyl functionality, enabling derivatization into amides, esters, fused ring systems, and fluorescent probes. These fluorescent derivatives are employed in bioanalytical chemistry and bioimaging for tracking and measuring biomolecular interactions.