Hesperadin is primarily used in scientific research as a selective inhibitor of Aurora B kinase, an enzyme involved in cell division. It is widely applied in studies related to mitosis, chromosome segregation, and cytokinesis. Due to its ability to disrupt proper cell division, hesperadin is used to investigate mechanisms of cancer cell proliferation and potential pathways for anticancer drug development. Researchers also utilize hesperadin in cell biology experiments to induce polyploidy and study cell cycle checkpoints. Its role in kinase inhibition makes it a valuable tool compound in signal transduction research and high-throughput screening for novel therapeutic agents.