GSK583 is primarily used as a potent and selective inhibitor of RIPK2 (Receptor-Interacting Serine/Threonine-Protein Kinase 2), making it a valuable tool compound in immunological and inflammatory research. It is widely applied in studies related to NOD1 and NOD2 signaling pathways, which play key roles in innate immune responses and are implicated in autoimmune diseases such as inflammatory bowel disease and rheumatoid arthritis. By inhibiting RIPK2, GSK583 helps suppress downstream NF-κB activation and cytokine production, allowing researchers to dissect the role of this pathway in disease models. Its selectivity and cellular activity make it suitable for in vitro and in vivo pharmacological studies aimed at validating RIPK2 as a therapeutic target. GSK583 is not intended for clinical use but serves as a critical probe in drug discovery and mechanistic biology.