Used in research to modulate the integrated stress response by selectively inhibiting GCN2 kinase, a key sensor of amino acid deprivation. This compound helps study cellular stress pathways, protein synthesis regulation, and immune responses. It has been applied in preclinical models to explore potential in enhancing cancer immunotherapy, improving cognitive function under stress, and managing neurodegenerative diseases. Its ability to fine-tune stress signaling makes it a valuable tool in cell biology and drug development.