GNF-5837 is primarily used in biochemical and pharmacological research as a selective inhibitor of certain kinase enzymes, particularly those involved in cell signaling pathways related to cancer progression. It has been investigated for its potential to suppress the activity of mutant forms of kinases that drive tumor growth, making it a valuable tool in the development of targeted cancer therapies. Due to its ability to penetrate cells and modulate specific signaling cascades, GNF-5837 is employed in studies aimed at understanding drug resistance mechanisms and identifying combination treatment strategies. Its application is largely confined to laboratory settings for in vitro and in vivo experiments, supporting early-stage drug discovery and validation of therapeutic targets.