GW5074 is primarily used in scientific research as a potent and selective inhibitor of c-Raf kinase, a key protein in the MAPK signaling pathway involved in cell growth and proliferation. It is widely applied in studies related to cancer biology, where it helps researchers investigate the role of Raf kinases in tumor development and progression. Due to its ability to cross the blood-brain barrier, GW5074 is also utilized in neuroscience research, particularly in models of neurodegenerative diseases such as Parkinson’s and Alzheimer’s, where it has shown neuroprotective effects by reducing oxidative stress and preventing neuronal cell death. Additionally, it has been explored for its potential in modulating mitochondrial function and inhibiting apoptosis in neuronal cultures, making it a valuable tool compound in both oncology and neuropharmacology research.