GSK650394 is primarily used in scientific research as a selective inhibitor of serum- and glucocorticoid-inducible kinase 1 (SGK1). It helps in studying cellular processes regulated by SGK1, such as ion transport, cell survival, and hormone signaling. Due to its ability to cross cell membranes effectively, it is valuable in cell-based assays to probe SGK1 involvement in disease models, particularly those related to inflammation, neurological disorders, and cancer. Researchers also use GSK650394 to investigate insulin resistance and metabolic pathways, as SGK1 plays a role in glucose homeostasis. Its application remains confined to laboratory and preclinical studies, with no current use in clinical therapy.