Dorsomorphin is widely used in biomedical research as a selective inhibitor of AMP-activated protein kinase (AMPK). It is particularly valuable in studies involving cellular energy homeostasis, as blocking AMPK activity allows scientists to investigate metabolic pathways and energy regulation in cells. Researchers also use dorsomorphin to suppress osteogenic differentiation in stem cell cultures, making it a tool in studies of cell fate and tissue development. Additionally, it has been applied in cancer research to explore the role of AMPK in tumor growth and proliferation. Due to its ability to cross cell membranes effectively, dorsomorphin is suitable for in vitro and cellular assays, though it is not typically used in clinical settings due to stability and selectivity limitations in vivo.