CHIR-98024 is primarily used in stem cell research as a potent and selective inhibitor of GSK-3 (glycogen synthase kinase-3). It supports the maintenance and self-renewal of pluripotent stem cells by activating the Wnt signaling pathway, which helps keep cells in an undifferentiated state. Due to its high selectivity, it is favored in protocols involving induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) where precise control of differentiation is required. It is also used in combination with other small molecules to improve reprogramming efficiency and direct cell fate decisions in regenerative medicine studies.