Used primarily in research settings as an irreversible inhibitor of the epidermal growth factor receptor (EGFR) tyrosine kinase. It is employed to study signaling pathways involved in cell proliferation and survival, particularly in cancer biology. Due to its ability to covalently bind to EGFR, it helps in understanding resistance mechanisms associated with EGFR mutations, including those found in non-small cell lung cancer. It is also utilized in preclinical studies to evaluate the efficacy of targeted therapies and to explore downstream effects of EGFR inhibition. Not intended for therapeutic use in humans.