Used primarily in research settings as an irreversible inhibitor of the epidermal growth factor receptor (EGFR) tyrosine kinase. It is employed to study signaling pathways involved in cell proliferation and survival, particularly in cancer cell lines. Due to its ability to covalently bind to EGFR, it helps in understanding receptor function and inhibition mechanisms. It has been utilized in preclinical studies to assess the effects of sustained EGFR blockade, especially in tumors with resistance to other EGFR inhibitors. Its application remains confined to laboratory research and is not used therapeutically in clinical practice.