CX-6258 HCl is primarily used in research settings as a potent and selective inhibitor of protein kinase C (PKC) isoforms. It serves as a valuable tool compound for studying PKC-mediated signaling pathways involved in cellular processes such as proliferation, differentiation, and apoptosis. Due to its inhibitory activity, it has been investigated for its potential in oncology research, particularly in models where aberrant PKC activity contributes to tumor growth. Additionally, CX-6258 HCl has shown utility in neuroscience research, where PKC modulation plays a role in synaptic transmission and neurodegenerative conditions. Its application remains confined to preclinical studies, aiding in target validation and mechanistic exploration of PKC-dependent diseases.