AG-1557 is primarily used as a selective inhibitor in biochemical research, particularly in studies involving kinase signaling pathways. It is employed to probe the function of specific tyrosine kinases, helping researchers understand their role in cell proliferation, apoptosis, and signal transduction. Due to its selectivity, AG-1557 is valuable in cancer research, where it aids in identifying potential therapeutic targets by modulating kinase activity in tumor cell lines. It is also utilized in early-stage drug discovery to validate the effects of kinase inhibition and assess pharmacological responses in cellular and enzymatic assays.