AG-490 is widely used in biomedical research for its ability to inhibit tyrosine kinases, particularly the JAK2 and EGFR pathways. It is employed to study signal transduction mechanisms involved in cell proliferation, apoptosis, and differentiation. Due to its inhibitory effect on JAK2/STAT3 signaling, AG-490 has been investigated in cancer research, showing potential in suppressing tumor growth in various leukemia and solid tumor models. It is also used to explore the role of tyrosine kinase activity in immune responses and inflammation. Its application extends to stem cell research, where it helps modulate signaling pathways to influence cell fate decisions. AG-490 is a valuable tool compound for validating therapeutic targets in oncology and immunology.