AMG-458 is primarily used in research settings as a potent and selective inhibitor of the epidermal growth factor receptor (EGFR). It is utilized to study signaling pathways involved in cell proliferation and survival, particularly in cancer cell lines that overexpress EGFR. Due to its high specificity, AMG-458 serves as a valuable tool in oncology research for evaluating the effects of EGFR inhibition on tumor growth and progression. It has been employed in preclinical studies to assess therapeutic strategies for cancers such as non-small cell lung cancer and other epithelial tumors. Its application also extends to investigating mechanisms of drug resistance and for combination therapy approaches with other targeted agents or chemotherapeutics.