Used primarily in research settings as a selective inhibitor of Mps1 (also known as TTK), a kinase involved in the spindle assembly checkpoint during cell division. By inhibiting Mps1, it disrupts proper chromosome alignment and segregation, leading to mitotic errors and cell death, particularly in rapidly dividing cells. This makes it a valuable tool for studying mitosis, chromosome stability, and potential anticancer strategies. It has shown activity in preclinical models against various cancer cell lines, especially when used in combination with other antimitotic agents, highlighting its potential as a candidate for targeted cancer therapy development.