ARP-100 is primarily used in research settings as a selective inhibitor of the Rho-associated protein kinase (ROCK) pathway. It has been applied in studies related to cell motility, cytoskeletal organization, and cancer metastasis due to its ability to modulate cellular contraction and migration. Its application extends to neuroscience research, where it aids in understanding neurite outgrowth and neuronal regeneration. ARP-100 is also utilized in cardiovascular research for investigating endothelial function and vascular tone regulation. Due to its specificity, it serves as a valuable tool compound in mechanistic studies involving ROCK signaling without significant off-target kinase effects.