A66 is primarily utilized in the field of medical research, particularly in the study of cancer. It acts as an inhibitor of the PI3K/AKT signaling pathway, which is crucial for cell growth and survival. By targeting this pathway, A66 helps researchers understand the mechanisms of cancer cell proliferation and survival, offering insights into potential therapeutic strategies. Its application extends to preclinical studies where it is used to evaluate the efficacy of inhibiting PI3K in various cancer models, aiding in the development of targeted cancer therapies. Additionally, A66 is employed in biochemical assays to investigate the role of PI3K in other cellular processes and diseases beyond cancer, contributing to a broader understanding of cell signaling pathways.