ERK5-IN-2 is primarily used in research settings to study the role of ERK5 (extracellular signal-regulated kinase 5) in various cellular processes. It is a selective inhibitor of ERK5, making it a valuable tool for investigating signaling pathways involved in cell proliferation, differentiation, and survival. Researchers utilize this compound to explore its potential in modulating diseases where ERK5 plays a critical role, such as cancer, cardiovascular disorders, and inflammatory conditions. By inhibiting ERK5, it helps in understanding the kinase's function and its impact on disease progression, aiding in the development of targeted therapies. Additionally, it is used in preclinical studies to assess the efficacy and safety of ERK5 inhibition as a therapeutic strategy.