Cot inhibitor 1 is primarily utilized in biomedical research to study and modulate signaling pathways involved in inflammation and immune responses. It is particularly effective in inhibiting the Cot/Tpl2 kinase, which plays a critical role in the activation of the MAPK pathway. This makes it a valuable tool for investigating conditions such as autoimmune diseases, cancer, and inflammatory disorders. Researchers use Cot inhibitor 1 to explore potential therapeutic strategies by blocking the downstream effects of this kinase, which can lead to reduced production of pro-inflammatory cytokines. Additionally, it aids in understanding the molecular mechanisms underlying immune cell activation and proliferation, providing insights into targeted drug development.