XMU-MP-1 is primarily utilized in biomedical research as a potent and selective inhibitor of the MST1/2 kinases, which are key components of the Hippo signaling pathway. This pathway plays a critical role in regulating cell proliferation, apoptosis, and organ size, making XMU-MP-1 a valuable tool for studying these processes. Researchers use it to investigate the effects of MST1/2 inhibition on tissue regeneration, cancer development, and immune responses. Its application has shown promise in promoting tissue repair and regeneration, particularly in liver and heart injury models. Additionally, it is employed in preclinical studies to explore its potential therapeutic benefits in diseases where the Hippo pathway is dysregulated, such as certain cancers and fibrosis.