WYE-687 is primarily utilized in biochemical research as a potent and selective inhibitor of the mTOR (mechanistic target of rapamycin) pathway. It is particularly effective in studying the role of mTORC1 (mTOR complex 1) in cellular processes such as growth, proliferation, and autophagy. Researchers employ WYE-687 to investigate its effects on cancer cell lines, as mTOR signaling is often dysregulated in various cancers. By inhibiting mTORC1, it helps in understanding the mechanisms of tumor growth and the potential for therapeutic interventions. Additionally, WYE-687 is used in studies exploring metabolic regulation and nutrient sensing, given mTOR's central role in these pathways. Its specificity makes it a valuable tool for dissecting complex signaling networks in both normal and diseased states.