WNK463 is primarily utilized in biochemical research to study the WNK signaling pathway, which plays a critical role in regulating blood pressure and electrolyte balance. It acts as an inhibitor of WNK kinases, making it a valuable tool for investigating diseases linked to these kinases, such as hypertension and renal disorders. Researchers use WNK463 to explore potential therapeutic strategies for conditions caused by dysregulation of ion transport in the kidneys. Its application extends to understanding the molecular mechanisms of WNK kinases in cellular processes, aiding in the development of targeted treatments for cardiovascular and kidney-related diseases.