WEHI-345 is primarily used in scientific research to study the signaling pathways involved in immune responses and inflammation. It acts as a selective inhibitor of RIPK2, a kinase that plays a critical role in the NOD signaling pathway. By inhibiting RIPK2, WEHI-345 helps researchers investigate the mechanisms underlying inflammatory diseases, such as Crohn's disease and other autoimmune conditions. Its application is crucial in developing potential therapeutic strategies targeting these pathways. Additionally, it is utilized in cellular and molecular biology studies to understand the broader implications of RIPK2 inhibition on immune regulation and cell survival.