VRK-IN-1 is primarily utilized in biochemical research as a selective inhibitor of vaccinia-related kinase (VRK) proteins. These kinases play a significant role in regulating cellular processes such as cell division, DNA damage response, and nuclear envelope dynamics. By inhibiting VRK activity, VRK-IN-1 helps researchers study the functional roles of these kinases in various biological pathways, particularly in cancer biology and cellular stress responses. Its application is crucial in understanding the molecular mechanisms underlying diseases and in identifying potential therapeutic targets. Additionally, VRK-IN-1 is used in experimental studies to explore its effects on cell proliferation and apoptosis, providing insights into its potential use in developing targeted cancer therapies.