Vps34-IN-2 is primarily utilized in research settings to study the role of Vps34, a class III phosphoinositide 3-kinase, in cellular processes. It is employed as a selective inhibitor to investigate autophagy, vesicle trafficking, and endosomal sorting. Researchers use this compound to explore its effects on cancer cell survival, as Vps34 is implicated in tumor growth and metastasis. Additionally, it aids in understanding the molecular mechanisms of neurodegenerative diseases, where autophagy dysfunction plays a critical role. Its application extends to studying immune responses, as Vps34 is involved in regulating immune cell signaling and function.