TG003 is primarily utilized in biochemical research as a potent inhibitor of CLK (CDC-like kinase) family proteins, which play a crucial role in the regulation of alternative splicing of pre-mRNA. Its application is significant in studying the mechanisms of splicing regulation and its implications in various diseases, particularly cancer and neurodegenerative disorders. Researchers employ TG003 to investigate the effects of splicing modulation on cellular processes, aiding in the development of potential therapeutic strategies targeting splicing-related pathologies. Additionally, it serves as a valuable tool in understanding the functional roles of CLK kinases in cellular signaling pathways.