STO-609 is primarily used in biochemical research as a selective inhibitor of calcium/calmodulin-dependent protein kinase kinase (CaMKK). It is particularly valuable in studies aimed at understanding the role of CaMKK in cellular signaling pathways, including those related to metabolism, cell growth, and neuronal function. Researchers utilize STO-609 to dissect the specific contributions of CaMKK in processes such as AMP-activated protein kinase (AMPK) activation, autophagy, and calcium signaling. Its application helps in elucidating the mechanisms underlying various physiological and pathological conditions, including cancer, metabolic disorders, and neurodegenerative diseases. Additionally, STO-609 serves as a tool to validate the involvement of CaMKK in experimental models, aiding in the development of targeted therapeutic strategies.