SB 202474 is primarily used in biochemical and pharmacological research as a negative control compound. It is structurally similar to p38 MAP kinase inhibitors but lacks the ability to inhibit the kinase itself. This makes it valuable in experiments to ensure that observed effects are due to specific kinase inhibition rather than non-specific interactions or off-target effects. Researchers utilize SB 202474 to validate the specificity of their findings, particularly in studies involving cellular signaling pathways and inflammation models. Its application helps in distinguishing between true pharmacological responses and artifacts, thereby enhancing the reliability of experimental results.