PIM-IN-1 is primarily used in biomedical research as a potent and selective inhibitor of PIM kinases, which are a family of serine/threonine kinases involved in cell survival, proliferation, and differentiation. Its application is significant in studying cancer biology, as PIM kinases are often overexpressed in various cancers, including leukemia, prostate cancer, and solid tumors. Researchers utilize PIM-IN-1 to investigate the role of PIM kinases in tumor growth and to evaluate its potential as a therapeutic agent in preclinical studies. Additionally, it aids in understanding signaling pathways and mechanisms of drug resistance in cancer cells, contributing to the development of targeted cancer therapies.