PI4KIII beta inhibitor 3 is primarily used in research settings to study the role of phosphatidylinositol 4-kinase beta (PI4KIIIβ) in cellular processes. It is a valuable tool for investigating lipid signaling pathways, particularly those involving phosphoinositides, which are crucial for membrane dynamics, intracellular trafficking, and signal transduction. By inhibiting PI4KIIIβ, this compound helps researchers understand its function in diseases such as cancer, viral infections, and metabolic disorders. It is also utilized in drug discovery efforts to identify potential therapeutic targets for conditions where PI4KIIIβ activity is dysregulated.