PHA-680632 is primarily utilized in biochemical research as a selective inhibitor of Aurora kinases, particularly Aurora A, B, and C. These kinases play a crucial role in cell division and mitosis, making PHA-680632 a valuable tool for studying cell cycle regulation and cancer biology. Researchers use it to investigate the effects of Aurora kinase inhibition on tumor growth, apoptosis, and mitotic processes. Its application extends to preclinical studies where it helps in understanding the potential therapeutic effects of targeting Aurora kinases in various cancers. Additionally, PHA-680632 is employed in high-throughput screening assays to identify and validate new anticancer compounds. Its role in elucidating the mechanisms of mitotic errors and chromosomal instability further underscores its importance in cancer research and drug development.