PF-562271 is primarily utilized in biochemical research as a potent and selective inhibitor of focal adhesion kinase (FAK). It is widely employed in studies aimed at understanding the role of FAK in cellular processes such as cell adhesion, migration, and survival. Researchers use this compound to investigate the signaling pathways involved in cancer progression, as FAK is often overexpressed in various tumor types. By inhibiting FAK, PF-562271 helps elucidate its contribution to tumor growth, metastasis, and resistance to therapies. Additionally, it serves as a valuable tool in preclinical studies to evaluate the potential of FAK inhibition as a therapeutic strategy for cancer treatment.